Year: 2014 | Month: March and June | Volume 2 | Issue 1 and 2

characterisation of Plant Growth Promoting Endophytic Bacteria Isolated from Lens culinaris Medic with Antagonistic Potential against Fusarium Oxysporum

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A total of 152 endophytic bacterial isolates belonging to Rhizobium, Pseudomonas and Bacillus spp. were isolated from the root nodules of Lens culinaris from seven different provinces of Uttar Pradesh (India) growing in different soil condition of varying pH 6.8-7.8. These isolates were characterized morphologically, physiologically and biochemically, and screened in vitro for their plant growth promoting traits such as production of indole acetic acid (IAA), ammonia (NH3), hydrogen cyanide (HCN), siderophore, phosphate solubilization and antifungal activity against Fusarium oxysporum. Only 27 endophytic showed positive results for all plant growth promoting attributes and antagonism against Fusarium oxysporum. All physio-biochemical characters including carbon utilization were analyzed by a Jaccard’s coefficient dendrogram, that classified the isolates into the three major groups viz., RT1, RT9-RT16, RT26 and RT27 isolates in one group, RT2-RT8, RT17-RT18, RT24 and RT25 isolates in second group, and RT19 - RT23 isolates in third group. On the basis of UPGMA cluster analysis and similarity with the standard strains these isolates were identified as Rhizobium strains RT1, RT9-RT16, RT26, RT27, Pseudomonas strains RT2-RT8, RT17-RT18, RT24 and RT25, and Bacillus strains RT19 - RT23. The strains RT1, RT2 and RT11 showed synergism among them and also found to possess plant growth promoting and antagonistic properties.

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