Year: 2017 | Month: December | Volume 5 | Issue 2
Differentiation of Multiple Shoots and Roots from Anther-Derived Callus Masses in Antirrhinum majus L.
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Abstract:
Anthers containing uninucleate pollen grains were cultured on MS and NB basal media supplemented with different auxins (IAA, NAA, 2,4-D) and cytokinins (BA and Kn) in various concentrations and combinations. MS medium was superior to NB medium in enhancing frequency of callus formation in anthers. Multiple shoots and roots were produced during subculture of callus on fresh medium supplemented with BA.
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