Year: 2014 | Month: March and June | Volume 2 | Issue 1 and 2

optimization of Fermentation Parameters for R3DSCc5 and R3DPMmP Strains for Ethanol Production

DOI:Coming soon...

Abstract:

Two newly isolated strains of Saccharomyces cerevisiae - R3DPMP and R3DSC5 were subjected to optimization studies with varying inoculum size, initial glucose concentration, initial pH and temperature using yeast extract peptone dextrose medium. Both the strains accumulated peak ethanol early with higher inoculum density and exhibited similar pattern with changing initial pH. Though higher glucose tolerance was noticed, 20% g/v and 25% g/v glucose concentration were found to be optimum for R3DPMP and R3DSC5 respectively. Similar amount of ethanol was accumulated within 30-400C temperature by R3DPMP and 30-350C temperature by R3DSC5. However, based on higher temperature tolerance, sugar tolerance and peak ethanol level, R3DSC5 appears to be superior to R3DPMP. Therefore, R3DPMP strain is recommended for brewery industry whereas R3DSC5 for both brewery and very high gravity fermentation.



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